Coding

Part:BBa_K4213004:Design

Designed by: Ioannis Retalis   Group: iGEM22_Thessaly   (2022-08-26)


PHT1;5 from A.thaliana with a linker for reporter gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 868
    Illegal EcoRI site found at 1452
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 868
    Illegal EcoRI site found at 1452
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 868
    Illegal EcoRI site found at 1452
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 868
    Illegal EcoRI site found at 1452
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 868
    Illegal EcoRI site found at 1452
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

added Kozak frame


Source

plant and linker from lab

References